Anubis was the patron of embalming. He was also the keeper of poisons and medicines. He provided unguents and rare herbs to help Isis and Nephthys with the embalming of Osiris. Anubis then performed the funeral of Osiris, which would be the model for all funerals to come. As he received the mummy into the tomb, he performed the 'Opening of the Mouth' ceremony.

In the "Hall of Maat", Anubis appears on behalf of the deceased. It was Anubis who saw that the beam of the great scale was in the proper position as he supervises the weighing of the heart of a deceased person against the feather of Maat. The god of knowledge,Thoth, records the results. It is also Anubis that protects the dead from Ammut, the 'Devourer'.